Composite

Part:BBa_K3089021:Design

Designed by: Aiai Dong   Group: iGEM19_Greatbay_SCIE   (2019-10-16)


T7 promoter+csgA-linker-mfp5-His fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 314
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 314
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 314
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A protein Linker(BBa_K3089019) was added between CsgA and mfp5 for direct fusion of functional domains without a linker may lead to many undesirable outcomes, including misfolding of the fusion proteins.


Source

CsgA was amplified from E.coli MG1655 genome and mfp5 was synthesized.

References