Composite
Part:BBa_K3089021:Design
Designed by: Aiai Dong Group: iGEM19_Greatbay_SCIE (2019-10-16)
T7 promoter+csgA-linker-mfp5-His fusion protein
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 314
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 314
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 314
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 314
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A protein Linker(BBa_K3089019) was added between CsgA and mfp5 for direct fusion of functional domains without a linker may lead to many undesirable outcomes, including misfolding of the fusion proteins.
Source
CsgA was amplified from E.coli MG1655 genome and mfp5 was synthesized.